The effect of hot and cold coffee brewing on light and dark roasts

cold brewing light dark roasts

Scientists from Thomas Jefferson University report that the content of potentially health-promoting antioxidants in coffee brewed without heat can significantly differ from a cup of coffee prepared with the same beans in a more traditional way, particularly for dark roasts.

“This study can inform coffee enthusiasts about how they may want to craft their own coffee at home, based on science and on analytical chemistry,” says Meghan Grim, an undergraduate student who worked on the project.

Researchers presented their results through the ACS SciMeetings online platform. A video summarising the research is available at

“After I first tried cold-brew coffee, I wanted to make it at home, but it didn’t turn out too great,” says Niny Z Rao, Ph.D., the project’s principal investigator. With her collaborator, Megan Fuller, Ph.D., Niny looked into the scientific literature to learn what she had done wrong.

“It turns out there weren’t any studies on cold-brew coffee at the time. So we decided to do some.”

In the cold brew process, ground coffee is mixed with room-temperature or colder water and steeped for as long as two days, sometimes in a refrigerator. It can be drunk cold or hot. In hot brewing, ground coffee is mixed with hot water and steeped at most for a few minutes. In either case, the coffee grounds are sometimes pressed, and the beverage is then filtered to remove the grounds.

The researchers previously assessed acidity and antioxidants in hot- and cold-brew coffee from lightly roasted beans. In another project, they analysed the effect of medium and dark roasting, and of medium and coarse grinding, on a few coffee attributes.

In this study, researchers roasted Colombian beans at five different temperatures — from light roasting at 174°C to dark roasting at 209°C — and studying the impact on acidity, antioxidants, and other natural chemicals in hot- and cold-brew coffee.

With the lighter roasts, Niny’s team finds that the content of caffeine and antioxidants is pretty similar in hot and cold brews. However, significant differences show up with the darker roasts.

“Hot brewing extracts more antioxidants from the grind than cold brew, and this difference increases with the degree of roasting,” Niny says.

She says this means hot-brew of dark roasts produces a potentially healthier drink. Hot brew also contains more of certain kinds of acids and total dissolved solids. The pH is about the same for both hot and cold brews at equivalent roast, though both become less acidic as roasting temperature increases.

“My advice to consumers has always been to drink what they like,” Niny says. “But if you want to craft a coffee beverage with antioxidants or acidity in mind, you may want to pay attention to roast. If you want a low-acid drink, you may want to use a darker roast. But remember that the gap between the antioxidant content of hot- and cold-brew coffee is much larger for a darker roast.”

However, Niny says one of the biggest challenges in all these projects is reproducibility.

“We have performed experiments in which we’re using the same beans, the same machine, the same settings, and it comes out not quite the same as the previous batch,” Niny says.

“The same goes for brewing. To create a cup that’s consistent every time is really difficult. I have a lot of respect for the baristas who can do that.”

To approach that level of perfection, researchers carefully standardised their processes. They developed a procedure for when the water should be added to the ground coffee, how to pour the water and for how long, how to shake the solution, how to press the brewed coffee, and how to analyse it. They set time limits for each step, with margins of just a few seconds. This rigor produced much more reproducible results.

The team has now begun comparing the impact of the two brewing processes and degree of roast on furans – flavour compounds present in raw coffee beans that are also generated through roasting. Such compounds are particularly important in cold-brew coffee, which doesn’t have the same distinct aroma as the hot drink because it lacks steam to carry volatile organic compounds to the nose.

The researchers thanked the United States Department of Agriculture’s National Institute of Food and Agriculture for support and funding.

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